This study aimed to extract apple juice from green apple fruits previously treated with aqueous extract of apple peels, before storing the extracted juice at a temperature of (4)°C, for a period of (30) days, and comparing it with the juice of untreated fruits in order to determine the effect of inhibiting the polyphenol enzyme. Polyphenol oxidase (PPO) in apple fruits according to the treatments mentioned in some characteristics of apple juice extracted from those fruits. The studied characteristics are: residual enzymatic activity, total soluble sugars, ascorbic acid content, total phenols content, and browning index, as they were immersed in Fruits treated with aqueous extract of apple peels had a significant effect in reducing the residual enzymatic activity and ascorbic acid values of the treated fruit juice, while the total soluble sugars and total phenols content had a significant increase in their values after the end of the storage period, while the browning index values were not affected at the end of storage.
Dias, C., Fonseca, A. M., Amaro, A. L., Vilas-Boas, A. A., Oliveira, A., Santos, S. A., ... & Pintado, M. (2020). Natural-based antioxidant extracts as potential mitigators of fruit browning. Antioxidants, 9(8), 715. https://doi.org/10.3390/antiox9080715
Paudel, P., Seong, S. H., Wagle, A., Min, B. S., Jung, H. A., & Choi, J. S. (2020). Antioxidant and anti-browning property of 2-arylbenzofuran derivatives from Morus alba Linn root bark. Food chemistry, 309, 125739. https://doi.org/10.1016/j.foodchem.2019.125739
Sukhonthara, S., Kaewka, K., & Theerakulkait, C. (2016). Inhibitory effect of rice bran extracts and its phenolic compounds on polyphenol oxidase activity and browning in potato and apple puree. Food chemistry, 190, 922-927. https://doi.org/10.1016/j.foodchem.2015.06.016
Chen, X., Ren, L., Li, M., Qian, J., Fan, J., & Du, B. (2017). Effects of clove essential oil and eugenol on quality and browning control of fresh-cut lettuce. Food Chemistry, 214, 432-439. http://dx.doi.org/10.1016/j.foodchem.2016.07.101
Hemachandran, H., Anantharaman, A., Mohan, S., Mohan, G., Kumar, D. T., Dey, D., ... & Ramamoorthy, S. (2017). Unraveling the inhibition mechanism of cyanidin-3-sophoroside on polyphenol oxidase and its effect on enzymatic browning of apples. Food Chemistry, 227, 102-110. https://doi.org/10.1016/j.foodchem.2017.01.041
Ruales, J., Baenas, N., Moreno, D. A., Stinco, C. M., Meléndez-Martínez, A. J., & García-Ruiz, A. (2018). Biological active ecuadorian mango ‘tommy atkins’ ingredients—An opportunity to reduce agrowaste. Nutrients, 10(9), 1138. https://doi.org/10.3390/nu10091138
Jirasuteeruk, C., & Theerakulkait, C. (2019). Ultrasound-assisted extraction of phenolic compounds from mango (Mangifera indica cv. Chok Anan) peel and its inhibitory effect on enzymatic browning of potato puree . Food Technology and Biotechnology, 57(3), 350. https://doi.org/10.17113%2Fftb.57.03.19.5728
Martínez-Hernández, G. B., Castillejo, N., & Artés-Hernández, F. (2019). Effect of fresh–cut apples fortification with lycopene microspheres, revalorized from tomato by-products, during shelf life. Postharvest Biology and Technology, 156, 110925. https://doi.org/10.1016/j.postharvbio.2019.05.026
Boonyaritthongchai, P., Supapvanich, S., Wongaree, C., Uthairatanakij, A., Jitareerat, P., Pongprasert, N., & Kaewmanee, N. (2017, September). Application of natural extracts from pineapple juice on inhibiting browning symptom of fresh-cut'Nam dok mai'mango. In IV Asia Symposium on Quality Management in Postharvest Systems 1210(pp. 235-240).
Supapvanich, S., Mitrsang, P., & Srinorkham, P. (2017). Effects of ‘Queen’and ‘Smooth cayenne’pineapple fruit core extracts on browning inhibition of fresh-cut wax apple fruit during storage. International Food Research Journal, 24(2), 559.
Parfait, K. K. K., Doudjo, S., Youssouf, K. Y. K., Mady, C., Emmanuel, A. N., & Benjamin, Y. K. (2022). Sensory Profile and Physico-Chemical Characteristics of Clarified and Pasteurized Cashew Apple Juice during Storage. Journal of Food and Nutrition Research, 10(3), 180-187.
Shomodder, A., Hossain, M. M., Das, P. C., Ranganathan, T. V., & Mazumder, M. A. R. (2021). Effect of Banana Leaf Extract, Pumpkin Seed Extract and Bee Honey Treatment on the Inhibition of Browning of Fresh Cut Guava. Journal of Agricultural Sciences (Sri Lanka), 16(2).
Rapeanu, G., Loey, V., Smout, C., and Hendrickx, M. (2005). Effect of pH on thermal and/or pressure inactivation of Victoria grape (Vitis vinifera sativa) polyphenol oxidase: a kinetic study. Journal of Food Science, 70(5), E301-E307. https://doi.org/10.1111/j.1365-2621.2005.tb09968.x
Sewwandi, S. D. C., Arampath, P. C., Silva, A. B. G., & Jayatissa, R. (2020). Determination and comparative study of sugars and synthetic colorants in commercial branded fruit juice products. Journal of Food Quality, 2020, 1-11. https://doi.org/10.1155/2020/7406506
Satpathy, L., Pradhan, N., Dash, D., Baral, P. P., & Parida, S. P. (2021). Quantitative determination of vitamin C concentration of common edible food sources by redox titration using iodine solution. Letters in Applied Bioscience NanoBioScience, 10, 2361-2369. https://doi.org/10.33263/LIANBS103.23612369
Agbor, G. A., Vinson, J. A., & Donnelly, P. E. (2014). Folin-Ciocalteau reagent for polyphenolic assay. International Journal of Food Science, Nutrition and Dietetics (IJFS), 3(8), 147-156
Hwang, C. C., Chien, H. I., Lee, Y. C., Lin, C. S., Hsiao, Y. T., Kuo, C. H., ... & Tsai, Y. H. (2023). Effect of High-Pressure Processing on the Qualities of Carrot Juice during Cold Storage. Foods, 12(16), 3107. https://doi.org/10.3390/foods12163107
SAS Institute. (2012). SAS/OR 9.3 User's Guide: Mathematical Programming Examples. SAS institute.
Azevedo, V. M., Dias, M. V., de Siqueira Elias, H. H., Fukushima, K. L., Silva, E. K., Carneiro, J. D. D. S., ... & Borges, S. V. (2018). Effect of whey protein isolate films incorporated with montmorillonite and citric acid on the preservation of fresh-cut apples. Food Research International, 107, 306-313. https://doi.org/10.1016/j.foodres.2018.02.050
Talasila, U. (2017). Preservation of cashew apple juice using Aloe vera and hydroxychavicol. International Journal on Nutraceuticals, Functional Foods and Novel Foods
Grobelna, A., Kalisz, S., & Kieliszek, M. (2019). The effect of the addition of blue honeysuckle berry juice to apple juice on the selected quality characteristics, anthocyanin stability, and antioxidant properties. Biomolecules, 9(11), 744. https://doi.org/10.3390/biom9110744
Malik Abdul Al-Kareem,Z and Hasan Ali Al-Jammaas,O . (2024). Inhibition of Polyphenol Oxidase Enzyme in Apple Slices using Aqueous Extract of Apple Peels. Al-Qadisiyah Journal For Agriculture Sciences, 14(1), 12-22. doi: 10.33794/qjas.2024.145520.1152
MLA
Malik Abdul Al-Kareem,Z , and Hasan Ali Al-Jammaas,O . "Inhibition of Polyphenol Oxidase Enzyme in Apple Slices using Aqueous Extract of Apple Peels", Al-Qadisiyah Journal For Agriculture Sciences, 14, 1, 2024, 12-22. doi: 10.33794/qjas.2024.145520.1152
HARVARD
Malik Abdul Al-Kareem Z, Hasan Ali Al-Jammaas O. (2024). 'Inhibition of Polyphenol Oxidase Enzyme in Apple Slices using Aqueous Extract of Apple Peels', Al-Qadisiyah Journal For Agriculture Sciences, 14(1), pp. 12-22. doi: 10.33794/qjas.2024.145520.1152
CHICAGO
Z Malik Abdul Al-Kareem and O Hasan Ali Al-Jammaas, "Inhibition of Polyphenol Oxidase Enzyme in Apple Slices using Aqueous Extract of Apple Peels," Al-Qadisiyah Journal For Agriculture Sciences, 14 1 (2024): 12-22, doi: 10.33794/qjas.2024.145520.1152
VANCOUVER
Malik Abdul Al-Kareem Z, Hasan Ali Al-Jammaas O. Inhibition of Polyphenol Oxidase Enzyme in Apple Slices using Aqueous Extract of Apple Peels. QJAS. 2024;14(1):12-22. doi: 10.33794/qjas.2024.145520.1152