Al-Qadisiyah Journal For Agriculture Sciences

Effect of Agricultural Exploitation on the Activity of Alkaline Phosphatase and Its Kinetic Properties in Some Soils

Authors

Luma Salih Jabbar Al-Taweel
Gaith Abedulkadhim Alwan Al- Jubouri

College of Agriculture / University of Al-Qadisiyah, Iraq

https://doi.org/10.33794/qjas.2019.162668
Abstract
In order to study the role of agricultural exploitation in the activity of Alkaline phosphatase in the province of Diwaniyah, six sites that differ in some of their chemical, physical and biological properties were selected (Diwaniyah, Sunniya, Shamiya, Daghara, Afak and Al Budair). Three types of soils were chosen in each location, namely, orchard soil, field soil and jungle soil. The kinetic parameters of the enzymes maximum velocity (Vmax) and Michaelis constant (Km) were estimated in all study soils using increasing concentrations of the controlled substance. The results present that the highest efficacy of this enzyme is in the orchard soil of all sites except the location of the center Diwaniyah and Shamia. The average efficacy values ranged between (208.11 - 234.95) μg P-Nitro phenol. g-1 soil. 1 hour-1. The highest value of maximum velocity (Vmax) recorded at the field soil of Al-Shamia site (108.57) μg P-Nitro phenol. g-1 soil. 1 hour-1. The lowest value recorded at the soil of a jungle field at the Sunniya location (49.62) μg P-nitro phenol. g-1 soil. 1 hour-1. While the orchard soil of Al-Budair had the highest value of the Michaelis constant (Km) of (85.90) mM, the orchard soil of the Sunniya location had the lowest value (24.34) mM. .

Keywords

agricultural exploitation
Asparaginase
Vmax
Michaelis constant
Al-Qadisiyah Journal For Agriculture Sciences
Volume 9, Issue 1
June 2019
Page 72-87

  • Receive Date 01 December 2019
  • Revise Date 20 December 2019
  • Accept Date 25 December 2019