Al-Qadisiyah Journal For Agriculture Sciences

The in Situ Effect of Phoenician Juniper Leaves on the Physicochemical and Microbiological Quality of Fermented Goat’s Milk Stored at Room Temperature

Document Type : Research Paper

Authors

1 Architecture and Environmental Heritage Laboratory (Archipel), Mohammed Tahri University of Bechar (08000), Bechar (Algeria). Faculty of Natural and Life Sciences, Department of Biology, Mohammed Tahri University of Bechar (08000),

2 Faculty of Natural and Life Sciences, Department of Biology, Mohammed Tahri University of Bechar (08000), Bechar (Algeria).

10.33794/qjas.2024.146685.1160

Abstract

This study aims to estimate the in situ effect of Phoenician juniper leaves on the physicochemical and microbiological quality of fermented goat’s milk stored under ambient conditions. In this study, we used two samples. The first sample was goat's milk flavored with juniper leaves, compared to the control sample without juniper leaves. We analyzed three physicochemical parameters: pH, titratable acidity, and temperature. Furthermore, the bacteriological analysis focused on the following parameters: Total aerobic mesophilic flora (TAMF), total coliforms (TC), fecal coliforms (FC), coagulase-positive Staphylococci (CoPS), and pathogenic bacteria (Salmonella spp and Listeria monocytogenes). The physicochemical results showed a change in the pH and titratable acidity of both samples with a higher acidity for the test sample compared to the control sample. This change was marked by an increase in a load of total aerobic mesophilic flora and lactic acid bacteria, a decrease in the total coliforms and thermotolerant coliforms, and the Staphylococci load in the test sample compared to the control sample, which experienced an increase in these bacterial parameters. For the test sample, juniper leaves reduced bacterial contaminants in fermented goat’s milk by 38% for total coliforms, 41% for fecal coliforms, and 89% for staphylococci. Both samples were free of Salmonella spp and L. monocytogenes. These results are probably due to the effect of bioactive compounds of Juniperus phoenicea L leaves known for their antimicrobial effect. This traditional practice of our ancestors can improve the quality of fermented goat’s milk stored even at ambient temperature.

Keywords

Main Subjects

References
  1. Leksir, C., Boudalia, S., Moujahed, N., Chemmam, M. (2019). Traditional dairy products in Algeria: Case of Klila cheese. J. Ethn Foods. 6: 7. https://doi.org/10.1186/s42779-019-0008-4
  2. Meribai, A., Ouarkoub, M., Bensoltane, A. (2016). Algerian dairy sector analysis : Deficit aspects and perspectives. J. New Sci. 37(7): 1986–
  3. Boumendjel, M., Feknous, N., Mekideche, F., Dalichaouche, N., Feknous, I., Touafchia, L., Metlaoui, N., Zenki, R. (2017). Caractérisation du lait de chèvre produit dans la région du Nord-Est Algérien. Essai de fabrication du fromage frais. Alger. J. Nat. Prod. 5(2): 492–506. https://doi.org/10.5281/zenodo.1098265
  4. Benyagoub, E., Bettache, G., Ayat, M., Benyagoub, R. (2018). Cost price/quality relationship of a strategic product and self-sufficiency challenge in the Algerian dairy sector: The case study of a local dairy industry. Econ. Agro-Aliment. 20(1): 55–74. https://doi.org/10.3280/ECAG2018-001004
  5. Benyagoub, E. (2023). An overview of Phoenix dactylifera date varieties in the province of Bechar (Southwest of Algeria): Productivity and challenges of the date palm sector. Al-Qadisiyah J. Agric. Sci. 13(2): 166–176. https://doi.org/10.33794/qjas.2023.145284.1150
  6. Benyagoub, E. (2022). Monitoring some physicochemical and bacteriological parameters and sensory analysis of Juniperus phoenicea leaves-supplemented goat milk: A Southwestern Algerian traditional flavored and fermented product. Asian J. Dairy Food Res. 41(2): 132−141. https://doi.org/10.18805/ajdfr.DRF-246
  7. Fernandez, A.B. (2019). Chemical composition of goat milk : Revision bibliografica. Arch. Vet. Anim. Sci. 1(1): 1–5. Retrieved from: https://escientificpublishers.com/chemical-composition-of-goat-milk-revision-bibliografica-AVAS-01-0007
  8. AlKaisy, Q.H., Al-Saadi, J.S., Al-Rikabi, A.K.J., Altemimi, A.B., Hesarinejad, M.A. Abedelmaksoud, T.G. (2023). Exploring the health benefits and functional properties of goat milk proteins. Food Sci. Nutr. 11(10): 5641– https://doi.org/10.1002/fsn3.3531
  9. Benyagoub, E., Ayat, M. (2015). Biochemical, physicochemical and microbiological properties of camel raw milk marketed in Bechar city (South-West of Algeria): Hygienic and safe consumers approach. Microbes Health. 4(1): 14–18. https://doi.org/10.3329/mh.v4i1.23087
  10. Benyagoub, E. (2022). Technological properties and antagonistic effect of lactic acid bacteria isolated from fermented goat milk flavoured with juniper leaves – a product from the southwest region of Algeria – against some microbial contaminants. NutraFoods. 2: 419−437. https://doi.org/10.17470nf-022-0051
  11. Benyagoub, E. (2023). Traditional goatskin churn (Chekoua): Quality of fermented goat milk flavoured with leaves of Phoenician juniper stored under ambient conditions. NutraFoods, 1: 472–484. https://doi.org/10.17470/NF-023-0056
  12. Benyagoub, E., Ayat, M., Belarbi, D. (2014). Physicochemical quality assessment of ultra-high temperature sterilized milk and milk products in Western Algeria. South Asian J. Exp. Biol. 4(3): 85–90. https://doi.org/10.38150/sajeb.4(3).p85-90
  13. Benyagoub, E., Guessas, B., Ayat, M., Benyagoub, R. (2017). Algerian dairy industry, Can it control bacterial contaminant indicators of hygienic quality deffects in the production chain of raw and pasteurized milk ? Int. J. Adv. Res. 5(1): 2237–2245. http://dx.doi.org/10.21474/IJAR01/3001
  14. ISO 8261 (2001). Milk and milk products-General guidelines for the preparation of test samples, initial suspension and decimal dilutions for microbiological examination. International Organization for Standardization.
  15. Ministry of Commerce (2014). Order of May 28, 2014 making the method of sample preparation, stock suspension, and decimal dilutions mandatory for microbiological analysis. (JORA n.38, 2014).
  16. AFNOR (1986). Analyses microbiologique. Tome I: Méthodes horizontales, contrôle de qualité des produits alimentaires. Association Française de Normalisation (AFNOR).
  17. NF V04-206 (1969). Lait-Détermination de l'acidité titrable. Norme Française.
  18. ISO/TS 11869 (2012). Fermented milks-Determination of titratable acidity-Potentiometric method.
  19. Ministère du Commerce (2004). Arrêté du 5 Safar 1425 correspondant au 27 Mars 2004 rendant obligatoire la méthode de dénombrement des organismes microbiens pour le lait fermenté. (JORA n.32, 2004).
  20. ISO 4833-2 (2013). Microbiologie de la chaîne alimentaire – Méthode horizontale pour le dénombrement des microorganismes - Partie 2: Comptage des colonies à 30°C par la technique d’ensemencement en surface.
  21. ISO 4832 (1978). Microbiologie-Directives générales pour dénombrement des coliformes-Méthodes par comptage de colonies obtenues à 30°C. Organisation Internationale de Normalisation.
  22. NF V08-050 (2009). Microbiologie des aliments-Dénombrement des coliformes présumés par comptage des colonies obtenues à 30°C. Association Française de Normalisation (AFNOR).
  23. Ministère du Commerce (2004). Arrêté du 26 Rajab 1425 correspondant au 11 septembre 2004 rendant obligatoire une méthode de contrôle microbiologique pour le lait pasteurisé (JORA n.70, 2004).
  24. ISO 6785 (2001). Milk and milk products-Detection of Salmonella International Organization for Standardization.
  25. Ministère du Commerce (2017). Arrêté du 8 Joumada El Oula 1438 correspondant au 5 février 2017 rendant obligatoire la méthode horizontale pour la recherche des Salmonella (JORA n.44, 2017).
  26. Corry, E.L., Curtis, G.D.W., Baird, R.M. (2003). Handbook of culture media for food microbiology, Part 2 Pharmacopoeia of culture media (Fraser broth and modified half Fraser broth), Prog. Ind. Microbiol. 37: 472–474.
  27. Ministère du Commerce (2006). Arrêté du 21 Chaâbane 1426 correspondant au 25 septembre 2005 rendant obligatoire la méthode de recherche de Listeria monocytogenes dans le lait et les produits laitiers. (JORA n.3, 2006).
  28. ISO 11290-1 (2017). Microbiology of the food chain-Horizontal method for the detection and enumeration of Listeria monocytogenes and Listeria Part 1: Detection method. International Organization for Standardization.
  29. Ministère du Commerce (2004). Arrêté du 4 Rabie Ethani 1425 correspondant au 24 mai 2004 rendant obligatoire une méthode de dénombrement des micro-organismes caractéristiques par une technique de comptage des colonies à 37°C dans le yaourt. (JORA n.43, 2004).
  30. Tille, M.P. (2018). Bailey & Scott’s diagnostic microbiology, 14th ed. Elsevier: 1136p.
  31. Benyagoub, E., Benchaib, S.M., Zaalan, A., Bendada, F. (2018). Prevalence and isolation of pathogenic strains responsible for some infections in Bechar’s community. Bangladesh J. Med. Sci. 19(3): 404−413. https://doi.org/10.3329/bjms.v19i3.45856
  32. Benyagoub, E., Mammeri, A. (2023). Physicochemical, biochemical, and microbiological quality of dried and salted camel meat (Kadid) from the Southwestern regions of Algeria. Fresen. Environ. Bull. 32(12): 3370–3386.
  33. Benyagoub, E. (2024). Methicillin, β-lactams, and clindamycin resistance profiles of Staphylococcus aureus strains isolated from patients with UTI in Bechar province (Algeria). Anti-infect agents. 22(1): e220823220161. https://doi.org/10.2174/2211352521666230822104016
  34. NA 2692 (1992). Milk and special milk for human consumption, Algerian standard.
  35. Ministère de l’économie (1993). Arrêté interministériel du 29 Safar 1414 correspondant au 18 Août 1993 relatif aux spécifications et à la présentation de certains laits de consommation. (JORA n.69, 1993).
  36. Ministry of Commerce (2017). Interministerial order of October 4, 2016, setting the microbiological criteria for foodstuffs. (JORA n.39, 2017).
  37. Nabbou, N., Benyagoub, E., Mellouk, A., Benchikh, A., Benabdrahmane, H., Hamouine, A. (2024). Chemical and biological treatments of dairy effluents using chemical coagulation process and selected bacteria in a laboratory-scale batch culture: Case of a dairy unit located in Bechar (Southwestern Algeria). Fresen. Environ. Bull. 33(2): 116–134.
  38. Adamu, J. (2021). Genetic and non-genetic (environmental) factors affecting milk yield and composition of small ruminant (A review). J. Agric. Econ. Environ. Soc. Sci. 7(1): 45–60.
  39. Magan, J.B., O’Callaghan, T.F., Kelly, A.L., McCarthy, N.A. (2021). Compositional and functional properties of milk and dairy products derived from cows fed pasture or concentrate-based diets. Compr. Rev. Food Sci. Food Saf. 20(3): 2769–2800. https://doi.org/10.1111/1541-4337.12751
  40. Freund, G. (1997). Intérêts nutritionnel et diététique du lait de chèvre. Paris : Ed Quae. Niort, 199p.
  41. Benyagoub, E., Guessas, B., Ayat, M., Sanebaoui, B. (2016). Physicochemical and bacteriological properties of some algerian traditional dairy products (Klila and Jben) marketed in Southwest of Algeria and their impact on consumers health. Int. J. Adv. Res. 4(2): 760–768. http://dx.doi.org/10.21474/IJAR01/2457
  42. Angioni, A., Barra, A., Russo, M.T., Coroneo, V., Dessi, S., Cabras, P. (2003). Chemical composition of the essential oils of Juniperus from ripe and unripe berries and leaves and their antimicrobial activity. J. Agric. Food Chem. 51(10): 3073– https://doi.org/10.1021/jf026203j
  43. Caudullo, G., de Rigo, D. (2016). Juniperus phoenicea in Europe: Distribution, habitat, usage and threats. In: San-Miguel-Ayanz, J., de Rigo, D., Caudullo, H., Houston Daurrant, T., Mauri, A. (Eds), European Atlas of Forest Tree Species. Publ. Off. EU, Lexembourg, pp. e012f63+.
  44. Draoui, M., Abdellah, M., Nora, N.B. (2020). Chemical compound and antibacterial activity of Juniperus phoenicea and Rosmarinus officinalis L. from Algerian Sahara. South Asian J. Exp. Biol. 10(2): 77–82. https://doi.org/10.38150/sajeb.10(2).p77-82
  45. Benyagoub, E., Nabbou, N. (2023). Screening phytochimique et évaluation de l’activité antibactérienne in vitro des extraits de feuilles de Juniperus phoenicea de la région de Aïn Sefra (Naâma-Algérie). Proceedings of the National Seminar on Biological Activities and Valorization of Animal and Plant Bioresources, March 1-2 (2023), Nour Elbachir University Center of El-bayadh, Algeria. pp.30. Id: T1P5.
  46. Benyagoub, E. (2023). Lactic acid bacteria isolated from fermented goat milk flavored with Phoenician juniper leaves stored in goatskin churn (Chekoua): A southwestern Algeria traditional by-product. Environ. Bull. 32(10): 3116–3135.
  47. Biadata, A., Szablewski, T., Lasik-Kurdys, M., Cegielska-Radziejewska, R. (2020). Antimicrobial activity of goat’s milk fermented by single strain of kefir grain microflora. Eur. Food Res. Technol. 246(2020): 1231– https://doi.org/10.1007/s00217-020-03483-2
  48. Moreno-Montoro, M., Navarro-Alarcon, M., Bergillos-Meca, T., Giménez-Martinez, R., Sanchez-Hernandez, S., Olalla-Herrera, M. (2018). Physicochemical, nutritional, and organoleptic characterization of a skimmed goat milk fermented with the probiotic strain Lactobacillus plantarum Nutrients. 10: 633. https://doi.org/10.3390/nu10050633.
Al-Qadisiyah Journal For Agriculture Sciences

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Available Online from 01 June 2024
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  • Receive Date: 07 February 2024
  • Accept Date: 12 March 2024
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