Document Type : Research Paper


1 Department of Biology, College of Science, Salahaddin University- Erbil, Iraq

2 Forestry department, Salahaddin University, Erbil- Iraq

3 Department of Biology, College of Science, Salahaddin University- Erbil, Iraq.


Nickel is an important and fundamental element for the growth and development of the plant. This study conducted to investigate the effect of different sized Ni nanoparticles (Ni NPs) with different concentrations on the physiological and anatomical characters of Brophyllum pinnatum seedlings. Four treatments used; 20 and 40 nm each with two concentrations; 0.20 and 0.40 ppm in four replications. Two doses of foliar application applied each after 30 days. The results showed that 40 nm/ 0.2 ppm spray had a promoter effect on plant height, shoot dry weight and chlorophyll content. While 20 nm/ 0.2 ppm act an inhibitor for all but stimulator for leaf area. Thus, adding an appropriate level of Ni NPs could improve general health, physiological growth and anatomical characters of Brophyllum pinnatum.


Main Subjects

  1. Vandebroek, I., Picking, D., Vandebroek, I. and Picking, D. (2020). Bryophyllum pinnatum (Lam.) Oken (Crassulaceae). Popular Medicinal Plants in Portland and Kingston, Jamaica, 61-70.
  2. Sanzari, I., Leone, A. and Ambrosone, A., 2019. Nanotechnology in plant science: to make a long story short. Frontiers in Bioengineering and Biotechnology, 7, 120.
  3. Adnan, M., Abbas, B., Asif, M., Hayyat, M.S., Raza, A., Khan, B.A., Hassan, H., Khan, M.A.B., Toor, M.D. and Khalid, M., 2020. Role of micro nutrients bio-fortification in agriculture: A review. International Journal of Environmental Sciences & Natural Resources, 24, 4, 209-213.
  4. Ghazanfar, S., Komal, A., Waseem, A., Hassan, W., Iqbal, R.J., Toor, S., Asif, M., Saleem, I.A., Khan, S.U., Tarar, Z.H. and Nazar, S. (2021). Physiological effects of nickel contamination on plant growth. NVEO-NATURAL VOLATILES & ESSENTIAL OILS Journal| NVEO, 13457-13469.
  5. Wang, H., Miao, L., Sun, X., Wu, L. and Fan, G. (2022). Experimental Study of Enzyme-Induced Carbonate Precipitation for High Temperature Applications by Controlling Enzyme Activity. Geomicrobiology Journal, 39, 6, 502-514.
  6. Manzoor, Z., Hassan, Z., Ul-Allah, S., Khan, A.A., Sattar, A., Shahzad, U., Amin, H. and Hussain, M., 2022. Transcription factors involved in plant responses to heavy metal stress adaptation. In Plant Perspectives to Global Climate Changes, 221-231, Academic Press.
  7. Zia-ur-Rehman, M., Anayatullah, S., Irfan, E., Hussain, S.M., Rizwan, M., Sohail, M.I., Jafir, M., Ahmad, T., Usman, M. and Alharby, H.F. (2022). Nanoparticles assisted regulation of oxidative stress and antioxidant enzyme system in plants under salt stress: A review. Chemosphere, 137649.
  8. Dappe, V., Dumez, S., Bernard, F., Hanoune, B., Cuny, D., Dumat, C. and Sobanska, S. (2019). The role of epicuticular waxes on foliar metal transfer and phytotoxicity in edible vegetables: case of Brassica oleracea species exposed to manufactured particles. Environmental Science and Pollution Research, 26, 20092-20106.
  9. Qadir, S. A. and Fathulla, C. N. (2023). Physiological and anatomical responses of common bean (phaseolus vulgaris l.) to nickle nanoparticles foliar spray. The Iraqi Journal of Agricultural Science, 54, 5.
  10. Miri, A.H., SHAkIb, E.S., EbRAHIMI, O. and Sharifi-Rad, J. (2017). Impacts of nickel nanoparticles on grow characteristics, photosynthetic pigment content and antioxidant activity of Coriandrum sativum L. Oriental Journal of Chemistry, 33, 3, 1297-1303.
  11. Bhalerao, S. A., Sharma, A. S., & Poojari, A. C. (2015). Toxicity of nickel in plants. International Journal of Pure and Applied Bioscience, 3, 2, 345-355.
  12. Sobati-Nasab, Z., Alirezalu, A. and Noruzi, P. (2021). Effect of foliar application of nickel on physiological and phytochemical characteristics of pot marigold (Calendula officinalis). Journal of Agriculture and Food Research,100-108.
  13. Wintermans JF, De Mots A. (1965). Spectrophotometric characteristics of chlorophylls a and b and their phenophytins in ethanol. Biochimica et Biophysica Acta (BBA)-Biophysics including Photosynthesis. 109, 2, 448-53.
  14. Raliya, R., Franke, C., Chavalmane, S., Nair, R., Reed, N., & Biswas, P. (2016). Quantitative understanding of nanoparticle uptake in watermelon plants. Frontiers in Plant Science, 7, 1288.
  15. Landa, P. (2021). Positive effects of metallic nanoparticles on plants: Overview of involved mechanisms. Plant Physiology and Biochemistry, 161, 12-24.
  16. Amde, M., Liu, J.F., Tan, Z.Q. and Bekana, D. (2017). Transformation and bioavailability of metal oxide nanoparticles in aquatic and terrestrial environments. A review. Environmental pollution, 230, pp.250-267.
  17. Jośko, I., Oleszczuk, P. and Skwarek, E. (2017). Toxicity of combined mixtures of nanoparticles to plants. Journal of hazardous materials, 331, 200-209.
  18. Moradbeygi, H., Jamei, R., Heidari, R. and Darvishzadeh, R. (2020). Investigating the enzymatic and non-enzymatic antioxidant defense by applying iron oxide nanoparticles in Dracocephalum moldavica L. plant under salinity stress. Scientia Horticulturae, 272, 109537.
  19. Mahmoud, A.W.M., Ayad, A.A., Abdel-Aziz, H.S., Williams, L.L., El-Shazoly, R.M., Abdel-Wahab, A. and Abdeldaym, E.A. (2022). Foliar application of different iron sources improves morpho-physiological traits and nutritional quality of broad bean grown in sandy soil. Plants, 11, 19, 2599.
  20. Berni, R., Luyckx, M., Xu, X., Legay, S., Sergeant, K., Hausman, J.F., Lutts, S., Cai, G. and Guerriero, G., 2019. Reactive oxygen species and heavy metal stress in plants: Impact on the cell wall and secondary metabolism. Environmental and Experimental Botany, 161, 98-106.