Document Type : Research Paper


1 Directorate of Planning, Ministry of Agriculture, Iraq.

2 Baghdad University Agriculture college

3 Office of Agricultural, Research Ministry of Agriculture, Iraq.


With the aim of current study to estimate genetic action, heritability percentage in its broad and narrow sense and inbreeding depression resulting from inbreeding, using the generation mean analysis method to analyze the generation's means of hybrids maize that different in flowering dates and maturity. The genetic action, that controls inheritance of the field traits to the selected inbred lines and their resulting hybrids using the generation mean analysis. Five pure inbred lines  of maize (ZA17WR, Zi17WZ, ZM74, ZM19 and ZM49W3E) were selected from fifteen different inbred lines with flowering dates and maturity in the spring season 2019 and were crosses according to the research  target (late × late),( late ×early) ,(early ×late) and( early ×early). On 2019 fall season were entered into a backcross program to analyze the means generation analysis. On 2020 spring season to produce the (six generations) which are (P1, P2, F1, F2, BC1 and BC2)   for the four crosses were evaluated in comparative experiments using a randomized complete block design (RCBD) and with three replicates.  Genetic analysis was done for the components of genetic variance for the three criteria, according to [1] for the traits  of the number of days to 75% tasselling, Number of days to 75% silking, plant height , ear, leaves area and yield of individual plant. Results showedThe dominance gene action is dominant in the inheritance of the silking and tassling   traits in the four crosses. As for the trait of the number of leaves and the yield of the plant, the dominance gene action controlling its inheritance was in the first hybrid and the additive gene action in the second, third and fourth crosses, while the  dominance action had the greatest contribution to the inheritance of the trait The area of leaves in hybrids 2, 3 and 4 and in the first hybrid was the largest additive gene action contribution, The percentages of heritability in the broad and narrow sense differed among the four hybrids for the traits studied. The highest percentage of heritability in the narrow sense for the trait of plant yield in the fourth hybrid was 91.50% due to the high additive variance, while the highest  percentage of heritability in the broad sense was for the trait of leaf area in the third hybrid was 99.27%The highest percentage of genetic improvement for the trait of the number of leaves in the fourth cross was 27.64% , and the highest Inbreeding Depression   for second generation F2 for yield in the same hybrid was 22.66%,


Main Subjects

[1]       Mather, k. and J. L. Jinks.(1982). Biometrical Genetics 3ᴿᵈ ed. Chapman and Hall, London pp: 396.
[2]       Hosptial F.(2001).Size of donor chromosome segments around introgressed loci  and reduction of linkage drag in marker –assisted backcross programs.Genetics:158:1363-1379. Schweitizer, J.A., Martinsen,
[3]       G.D.Whitham, T.G.(2002).Cotton wood hybrids gain fitness traits of both parents: a mechanism for their long – term persistence .American Journal of Botany .89(6):981-990.
[4]       Al-Amiri, A.M.J.(2021).Detection of non-allelic interaction via generation mean analysis for factorial hybrids maize at different locations. Ph.D. Dissertation, Dept. of Field Crop, Coll. Of Agricultural Engineering Science, University of Baghdad.  pp: 191.
[5]       Mariani ,L .and A.Ferrante.(2017).Agronomic management for enhancing plant tolerance to a biotic stresses-drought,salinity,hypoxia,and lodging.Horticulturae,3(52): 1-18.doi:10.3390/ horticulturae 3040052
[6]       Hasan, A, A, M. (2005). Improve quantities traits and biological statically and applications in plant breeding program .Cairo. Egypt .
[7]       Ali,A.,H.Rahman,Farhatullah and Z.Shah.(2019).Genetic Analysis of Yield and its Contributing Traits in Maize. Zea.mays L. Sarhad.J.of Agri.35(2):654
[8]       Changji, H.; J. Woongcho and T. yamakawa.(2006). Diallel analysis of plant earlength in tropical maize (Zea mays L.). J. Fac. Agric Kyushu Univ.51(2): 237-236.
[9]       Iqbal, M.( 2009). Genetic analysis of maturity and yield attributes in subtropical maize. Ph.D. Thesis, Dep. of Plant Breeding and Genetics, Fac. of Crop Production Sci., NWFP Agricultural Univ., Peshawar, Pakistan.
[10]   Hussain , L. (2009) Genetic of drought tolerance in maize. Ph. D. thesis, University of Agriculture. Dhward, India.
[11]   Anis, A. H. A.( 2010) Estimation of genetic parameters in maize (Zea mays L .). Using single and triple hybrids, Ph.D. thesis, College of Agriculture and Forestry, University of Al Mosul
[12]   Iqbal, M . K., H . Rahman , I . H . K. Sher and J . Bakht .( 2010) . Heterosis for morphological traits in subtropical maize (Zea mays L.) maydica 55:41–48 .
[13]   Sher, H., M. Iqbal, K. Khan, M. Yasir and H. Ur-Rahman. 2012. Genetic analysis of maturity and flowering traits in maize (Zea mays L.). Asian Pacific Journal of Tropical Biomedicine, 621-626.
[14]   Al-Hamdani, Z. B. Fathi. 2012. The nature of gene action in complete cross of maize, PhD disselation , College of Agriculture and Forestry, University of Mosul.
[15]   Shahrokhi, M., S. K. Khorasani, and A. Ebrahimi.( 2011). Generation mean analysis for yield and yield component in maize (Zea mays L.). J. of plant Physiology and breeding (12): 59-72.
[16]   El-Mouhamady, A. A., A. A. Abdel-Sattar, E. H. El-Seidy and H. A. Abo-Yousef. (2013). Genetic classification for salinity tolerance in some promising lines of maize (Zea mays L.). J. Appl. Sci. Res., 9(1): 298-308
[17]   Kannosh, O. A.2014. Genetic analysis of some physiological traits, yield and components of yellow maize using cross-crossing. Master Thesis. Department of Field Crops - College of Agriculture - University of Anbar.pp:95.
[18]   Kahriman.F.C.O.Egesel,.R.Cebeci,A.Demir,and Sara bayrakar.2015.Genetic Analysis of flowering in Maize based on Calendar and Thermal Time.YYUJ.Agr.Sci;25(2):193-199.
[19]   Wuhaib, K.M, B.H.Hadi and W.A.Hassan.2017 Estimation of genetic variation components, average degree of dominance and heritability for several traits of maize in four crosses. J.Agri Veterinary Sci 10(10):53-57.
[20]   Hassan, W.A., B.H.Hadi, M.SH. Hamadalla. 2020. Study the GCA and SCA effects of five inbred lines of maize according to half diallel mating system. Al Qadisiyaha J.Agri.Sci 10(2):343-348
[21]   Saleh, H.S. and I.Salman. 2005. Posted on the recommended fertilizer Varieties according to the fertilizers available for summer and winter crops. Ministry of Agri.central Fertilizer syntheses committee.
[22]   Ministry of Agriculture .2006. Guidelines for the cultivation and production of sorghum. The General Authority for Agricultural Extension and   Cooperation. Sorghum research development project. Guidance Bulletin No. 19
[23]   Elsahookie,M.M.m and A. A. Dawoood.2021 . Genome and plant breeding. Ministry of Agriculture - Republic of Iraq.PP:150.
[24]   El sahookie, M.M. 2009. Seed Growth Relationships. Coll.Of Agric.Univ .of Bag hdad. Ministry of Higher Edu and Res. Pp: 150
[25]   Elsahookie, M. M. 1985. Shortcut method for estimating plant leaf area in maize . J. Agron . and Crop Sci.154:157-160.
[26]   Agrwal, V. and Z.Ahmed.1982. Heritability and genetic advance in Triticale IndianJ.Agric.Res.16 (4):19-23
[27]   Singh, R. K. and B. D. Chaudhary. 1977. Biometrical method in quantitative genetic analysis. Kamla Nagar. Delhi 110007. India
[28]   Burton, G. W. 1951. Quantitative inheritance in pearl millet (Pennisetum glaucum). Agro. J., 43: 409-417.
[29]   Abd, N. Y. 2012. Estimation of Action and Gene Number in some growth traits in maize (Zea mays L). . The IraqiJ.Agri.Sci.43(1):49-57
[30]   Abdel amir,A.N.,and  B.H.Hadi .2018. Evaluate the performance of double single hybrids and inbreds of maize under different plant population and estimate Heterosis and hybrid vigor.)Yield and its components).
[31]   Alabd AlHadi ,Msbbouah and S.AL-Ahmad.2014.Heterosis and inbreeding depression in population of maize(Zea may L). Damascus University Journal of Agricultural Sciences. Volume 30. Issue( 2) : 95-112
[32]   Al-Khafaji, M. J, Elsahookei.M, M, and Jiyad.S.J. 2012. Formula for a forecast ratioGenetic rotation in the F2 population of jalapenos. Iraqi Agricultural General Assembly,: 1(4):.9-1
[33]   Pacheco, C. A., M. X. Santos, C. D. Cruz, S. N. Parentoni, P. E. O. Guimaraes, E. E. G. Gama, A. E. da Silva, H. W. L. de Carvalho and P. A. V. Junior.( 2002) . Inbreeding depression of 28 maize elite open pollinated varieties . Genetics and Molecular Bio, 25(4): 441 – 448 .
[34]   Amanah, A. J.  and  B.H.Hadi.2021. Genetic Analysis by Using Partial Diallel Crossingof Maize In High Plant Densities (Estimation GCA, SCA and Some Genetic Parameters). Earth and Environmental Science 910,. doi:10.1088/1755-1315/910/1/012135
[35]   Srdic, J., A.Niktic and Z.Pajic.2008.SSR markers in characterization of sweet corn inbredlines.Gentika.40 (2):169-177.
[36]   Mehboob, A., S. Khan, F. Ahmad, N. H. Shah and N. Akhtar. (2010). Evaluation of 99 S1 lines of maize for inbreeding depression. Pak. J. Agri. Sci., 47(3): 209-213.
[37]   Wannows,A.A.M.Y .Sabbouh, and S.A.Al-Ahmad( 2015).Generation mean analysis technique for determining genetic parameters for some quantitative traits in two maize hybrids(Zea mays L)Jordan J.of Agric.Sci.11(1):59-72 .
[38]   Kumar, S., U., Chandel, S. K., Guleria and R., Devlash.(2019). Combining ability and heterosis for yield contributing and quality traits in medium maturing inbred lines of maize (Zea mays L.) using line x tester. IJCS, 7(1), 2027-2034.‏
[39]   Maiolia,M.F.D, R.J.B Pinto, T.A.dasilva, D.A.Rizzaardi, R.A.Matsuzaki, M.A.Sato, T.G.Eiseleand, G.D.Lago Garcia. (2021). Partial diallel and genetic divergence analysis in maize inbred lines. Acta Sci. ,Argon, Vol.43 (20) :1-11
[40]   Al Bethni H.H, B.H.Hadi and H.M Kareem.(2022). Genetic analysis of flowering and physiological maturity characteristics by Generation Mean Analysis (GMA) of four hybrids of maize (field traits). NeuroQuantology 20(5):3744-3763
[41]   Arnhold, E., D. J. H. Da Silva, O. L. De Mello Filho and J. M. S. Viana. (2007). Inbreedingdepression simulation in popcorn cultivars to estimate the effective population size forgermplasm conservation. Crop Breeding and Applied Biotechnology, 7: 87-93.
[42]   Roff, D. A.( 2002). Inbreeding depression: tests of the over-dominance and partial dominance hypotheses. Evolution, 56: 768-775.
[43]   43) Woyengo. V.W., OM., Odongo and S. Aganga.(2008). Analysis combining ability for (72) pure line of maize (Zea mays L.) and development itshybrids by topcroos to resistance the leaf disease. J. Agron. Sci. 50(10)710-740.
[44]   Saied, A.A.A.( 2009.) Estimating the strength of some genetic parameters using partial hybridization in maize (Zea mays L.) M.Sc Thesis, college of Agri,Univer. Of Mosul.Pp: 59 .
[45]   Abed, N.Y., B.H.Hadi, W.A.Hassan and K.M.Wuhaib. 2017. Assessment yield and its components of Italian Maize inbred lines by full diallel cross. AL-Anbar J. of Agric. Sci.(special No. of 5th Sci. conf. of the Faculty of Agric. Univ. of Anbar (part 1), 15: 1114-128.
[46]   Mesribet, N. A. and H. J. H. Al-Dulaimi.(2017).  Estimation of hybrid vigor, combining  ability and expected genetic improvement in maize using  half diallel  crossing. Anbar Journal of Agricultural Sciences, 15(2), 408-417.
[47]   Hammadi, H. J., and A.A., Abed.(2018). Determination heterosis, combining ability and gene action using half diallel crosses in maize. The Iraqi Journal of Agricultural Science, 49(6), 954.‏