Document Type : Research Paper

Authors

1 Agronomy department, Mouloud Mammeri University, Tizi-Ouzou, Algeria

2 Agronomic sciences, biological sciences and agronomic sciences, mouloud mammeri university, Tizi-Ouzou, Algeria

3 Department of Agricultural Sciences, Faculty of Biological Sciences and Agronomic Sciences, University of Mouloud MAMMERI, Tizi-Ouzou, Algeria.

Abstract

The antibacterial action of the carob tree offers the possibility of using it for the disinfection of treated wastewater in biological process wastewater treatment plants. This paper reviews studies conducted to investigate the effect of carob tree on the reduction of indicator bacteria of fecal contamination that cannot be removed after the secondary stage of biological treatment. The aim is to use the wastewater safely in irrigation and expand the list of irrigable plants with this unexploited resource. Physico-chemical and bacteriological parameters were used to determine the optimal conditions and evaluate the treatment’s efficiency. A contact time of 1 h and a dose of 1 mL of disinfectant reduced E. coli and fecal coliforms from 5.105 CFU to 1 237 CFU (99.75%) and from 7.106 CFU to 1 505 CFU (99.98%), respectively. For the same contact time, the fecal streptococci were reduced from 4.105 CFU to 59 CFU (99.99%). According to WHO standards, this dose and contact time are sufficient to use this water for irrigation. A minimum contact time of 2 h with a dose of 1 mL of the aqueous extract of the green pod of the carob is required to obtain a reduction of E. coli and fecal coliforms from 5.105 CFU to 5 254 CFU (99.95%) and from 7.106 CFU to 340 CFU (99. 99%) to meet the Algerian standards of reuse of treated wastewater being more stringent. The results show that the same conditions reduce fecal streptococci from 4.105 CFU to 5 CFU (99.99%). This elimination level shows that green carob juice can be used for disinfection with the necessary doses for each bacterial load and sufficient contact time.

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Main Subjects

[1]World health Organization (WHO). Guidelines for the safe use of wastewater, excreta and grey water:    Wastewater use in agriculture, Geneva Switzerland, 2006; 2: 2019 p.
[2]Bouabdelli M. S. (2021). Caractérisation spatio-temporelle et prévision de la sécheresse dans le Nord de l’Algérie (Doctoral dissertation, ECOLE NATIONALE SUPERIEURE D’HYDRAULIQUE).
[3]Djaffar S. & Kettab A. (2018). La gestion de l’eau en Algérie: quelles politiques, quelles stratégies, quels avenirs?. Algerian Journal of Environmental Science and Technology, 4(1).
[4]Lamaddalena R. & Frascari D. (2020). Développement et mise à l’échelle des technologies de traitement et de réutilisation des eaux usées dans les pays d’Afrique méditerranéenne: le projet MADFORWATER.
[5]Office National d’Assainissement (ONA). Document interne, (2019).
[6]Vandermeersch S. (2006). Étude comparative de l’efficacité des traitements d’épuration des eaux usées pour l’élimination des micro-organismes pathogènes. Université libre de Bruxelles.
[7]Bouti C., Héduit A. & Helmer J. M. (2009). Technologies d'épuration en vue d'une réutilisation des eaux usées traitées (REUT) (Doctoral dissertation, irstea).
[8]Chaouki A. C. H. A. C. H. A. & Messaouda A. L. B. A. R. (2021). Utilisation de la poudre des noyaux du caroube (Ceratoniasiliqua) comme bio-coagulant pour le traitement des eaux potables (Doctoral dissertation).
[9]Santonocito D., Granata G., Geraci C., Panico A., Siciliano E. A., Raciti G. & Puglia C. (2020). Carob Seeds: Food Waste or Source of Bioactive Compounds?. Pharmaceutics, 12(11), 1090.
[10]       Hsouna A. B., Trigui M., Jarraya R. M., Damak M., & Jaoua S. (2015). Identification of phenolic compounds by high performance liquid chromatography/mass spectrometry (HPLC/MS) and in vitro evaluation of the antioxidant and antimicrobial activities of Ceratonia siliqua leaves extracts. Journal of Medicinal Plants Research, 9(14), 479-485.
[11]        Makris D. P. &  Kefalas P. (2004). Carob pods (Ceratonia siliqua L.) as a source of polyphenolic antioxidants. Food Technology and Biotechnology, 42(2), 105-108.
[12]       TABET F. (2014). Activité antimicrobienne des extraits phénoliques de caroube Ceratoniasiliqua (L) (Doctoral dissertation, Université de Mostaganem-Abdelhamid Ibn Badis).
[13]       Darwish W. S., Khadr A. E. S., Kamel M. A. E. N., Abd Eldaim M. A., El Sayed I. E. T., Abdel-Bary H. M. & Ghareeb D. A. (2021). Phytochemical Characterization and Evaluation of Biological Activities of Egyptian Carob Pods (Ceratonia siliqua L.) AqueousExtract: In Vitro Study. Plants, 10(12), 2626.
[14]       Rodier J.,  legube B., Merlet N., Analyse of water (9th ed. Paris: Dunod, 2009).
[15]       American public Health Association (APHA). Standard methods for the examination of water and wastewater. (21st ed. Washington (D.C.): American Water Works Association and Water and Environment Federation, 2005).
[16]       NgandjuiTchangoue Y. A., DjumyomWafo G. V., Wanda C., Soh Kengne E., Kengne I. M. & KouamFogue S. (2019). Use of Moringa oleifera seed extracts to polish effluents from natural systems treating faecal sludge.  Environmental technology, 40 (15), 2018-2026.
[17]       Joslyn M. A., Nishira H. & Ito S. (1968). Leucoanthocyanins and related phenolic compounds of carob pods (Ceratonia siliqua). Journal of the Science of Food and Agriculture, 19(9), 543-550.
[18]       El Ansari Zineb B. M., Alain B. & Ahmed L. (2017). Total polyphenols and gallic acid contents in domesticated carob (Ceratonia siliqua L.) pods and leaves. Int J Pure App Biosci, 5(4), 22-30.
[19]       Nishira H. & Joslyn M. A. (1968). The galloyl glucose compounds in green carob pods (Ceratonia siliqua).  Phytochemistry, 7(12), 2147-2156.
[20]       Abi Azar R. (2007). Milk protein complexation by green carob pods extract. Technologicalproperties of obtained coagulums (Doctoral dissertation, AgroParisTech).
[21]       Institut Algérienne de Normalisation  (IANOR). (2014). Protection de l'environnement Réutilisation des eaux usées épurées à des fins agricoles. municipales et industrielles Spécifications physico-chimiques et biologiques, AVPN 17683:2014 Edition : 01 IANOR.
[22]       Shao D., Li J., Tang R., Liu L., Shi J. & Yang H. (2015). Inhibition of gallic acid on the growth and biofilm formation of Escherichia coli and Streptococcus mutans. Journal of food science, 80(6), M1299-M1305.