Document Type : Research Paper


1 Faculty of Agriculture, University of Al-Qadisiah, Iraq.

2 Faculty of Agriculture, University of Aleppo, Syria.


This research was carried out in the Research Center of the Faculty of Agriculture in Al-Muslimiya, 240 hybrid Babcock B300 female laying hens were randomly distributed since the first day of the experiment into four independent groups. Each group included 60 birds according to three replicates (20 birds in each replicate). Lactic acid bacillus was added to the feed mixture according to three levels (50 mg/kg; B group, 100 mg /kg; C group and 150 mg/kg; D group), while the first group was considered as a control group (A group) and was without additions. During the production period, the results showed that the addition of lactic acid bacillus led to significant differences (p<0.03) in the egg production and shell thickness compared with the control group. The additions did not affect the egg weight and feed consumption rate as well as the feed conversion ratio. The birds of the third group (C group) showed clear superiority in various productive traits compared to others in the other groups throughout the productive period followed by the birds of group D and then group B. The current study concluded that adding 100 mg /kg lactic acid bacillus to the feed mixtures for laying hens will contribute to raising the productive efficiency of laying hens and improving egg specifications.


Main Subjects

[1]     Mardenli, O. 2021. The Progeny test of Friesian sires for milk traits by using the contemporary comparison method. Rev Colombiana Cienc Anim. Recia. enero-junio; 13(1):e747.
[2]     Mohammad, M. S., Mardenli, O., Al-TAwash, A.S.A.2021. Evaluation of The Cryopreservation Technology of Poultry Sperm: A Review Study. IOP Conf. Series: Earth and Environmental Science 735 (2021) 012016
[3]     Al-Kerwi Mohammad, M.S., Amin Al-Tawash, A.S., Salah Almrsomi, T.2020. Comparison the Effect of Adding Waterly Extract of Black Seeds (Nigella Sativa ), Chamomile and Fenugreek with Drinking Water on Productive Performance for Broiler. IOP Conference Series: Earth and Environmental Sciencethis, 553(1), 012043
[4]     AL-Tawash, A.S.A., Suwaihi, T.A., Mohammad, M.S., Mohsen, A.A.2020. Beneficial effects of chrysin and ginger on blood biochemical parameters, hematology and immune response of broilers. Plant Archivesthis , 2020, 20, pp. 215–219
[5]     Almrsomi, T.S., Areaaer, A.H., Mohammad, M.S.2021. Influence of addition different levels of ginger powder in diet on productive performance of broiler ross. Indian Journal of Ecologythis , 2021, 48, pp. 6–9
[6]     Collignon, P.J., 1999- Vancomycin – resistant entrococci and use of avoparcin in animal feed : Is there alink ? Med .S. Aust .171: 144-146.
[7]     Thorns, C.J., 2000- Bacterial food – borne Zoonoses . Revue Scientifque et technique . 19:226-239.
[8]     Sahin, O. ,T.Y. Morislita , and Q. Zhang .2002- Camylobacter Colonization in Poultry: Sources of infection modes and transmission .Anim. Health Res. Rev ., 3:95-105.
[9]     Verstegen , M.W., and B.A. WIlliams ., 2002- Alternatives to the use of antibiotics as growth promoters for monogastric animals . Anim. Biotechnol 13:113-27.
[10]  Griggs, J. P. and J. P. Jacob., 2005- Alternatives to antibiotics for organic poultry production. J. Appl. Poult. Res. 14:750-756.
[11]  Haddadin, M. S. Y.; S. M. Abdulrahim; N. H. Odetallah AND R. K. Robinson., 1997- A proposed protocol for checking suitability of Lactobacillus acidophilus cultures for use during feeding trials with chickens. Tropic. Sci. 37: 16-20.
[12]  Hayirlı A, N. Esenbuğa, M. Macit, M. A. Yörük, A. Yildiz and H. Karaca., 2005- Nutrition practice to alleviate the adverse effects of stress on laying performance, metabolic profile and egg quality in peak producing hens: II. The probiotic supplementation. Asian-Aust. J. Anim. Sci.. Vol 18, No. 12 : 1752-1760.
[13]  Yousefi.M and K.Karkoodi., 2007-Effect of Probiotic and Saccharomyces cerevisiae Supplementation on performanace and Egg Quality of Laying Hens.,International of Poultry Science 6(1):52-54.
[14]  Panda, A. K., Rama Rao, S. S., Raju, M. V., & Sharma, S. S. (2007). Effect of probiotic (Lactobacillus sporogenes) feeding on egg production and quality, yolk cholesterol and humoral immune response of White Leghorn layer breeders. Journal of the Science of Food and Agriculture, 88(1), 43–47. doi:10.1002/jsfa.2921
[15]  Nahashon, S. N.; H. S. Nakaue and L. W. Mirosh., 1994- Production  variables and nutrient retention in single comb white leghorn laying pullets fed diets supplemented with direct fed microbial. Poultry Sci. 73:1699-1711.
[16]  Mohan B., Kadirvel R., Bhaskaran M., Natarajan A., 1995- Effect of probiotic supplementation on serum yolk cholesterol and egg shell chickens in layers, Br. Poult. Sci., 36: 799-803.
[17]  Balevi, T., U.S. Uçan, B. Coşun, V. Kurtoğu & İ.S. çetingül (2001) Effect of dietary probiotic on performance and humoral immune response in layer hens, British Poultry Science, 42:4, 456-461, DOI: 10.1080/00071660120073133
[18]  Hunton, P., 2004-America’s Leading. World Poultry Congress Hears Research Reports. p: 1-4.
[19]  Siam S. M. and Alm El-Dein A. K., 2004- Influence of using two different levels of probiotics on egg performance, blood and yolk cholesterol of laying hens. Egyptian Poultry Science Journal , 1110-5623.2004.
[20]  Zangana, B. S. R and Al-Hajo, N. N. A., 2009- A comparative study on the effect of locally produced probiotic, prebiotic and synbiotic on egg chemical composition and egg serum cholesterol of White Leghorn hens.  Journal Egyptian Journal of Nutrition and Feeds. 12:(1). 177-185.
[21]  Alkhalf A., M. Alhaj and I. Al-Homidan., 2010-. Influence of probiotic supplementation on blood parameters and growth performance in broiler chickens. Saudi Journal of Biological Sciences 17(3): 219-225.
[22]  Tortuero, F., Fernández, E., 1995- Effects of inclusion of microbial cultures in barley-based diets fed to laying hens. Anim. Feed. Sci. Tech. 53:255-265.
[23]  Abdulrahim A; M. S. Y. Haddadin A; E. A. R. Hashlamoun A; R. K. Robinson., 1996- The influence of lactobacillus acidophilus and bacitracin on layer performance of chickens and cholesterol content of plasma and egg yolk. British Poultry Science. 37(2): 341-346.
[24]  Lehmood, B. A. M., Hadi, M. M. 2013.Effect of humic acid and rosemary on productive and economical traits of Japanese quail birds grown in hot environment. Euphrates Journal of Agriculture Science, 5(4:):64-75
[25]  SAS  Institute  Inc.  2017.  SAS/STAT® 14.3 User’s Guide. Cary, NC: SAS Institute.
[26]  Chen, Y.C. and T.C. Chen., 2003- Effects of commercial probiotic or prebiotic  supplementation on production, size and quality of hens egg. Poult. Sci., 82: 330.
[27]  Bozkurt Mehmet., Küçükyilmaz Kamil., Ayhan Veysel., Çabuk Metin., Uğur Çatli Abdullah., Erbeyli Tavukçuluk, 2011- Performance of layer or broiler breeder hens varies in response to different probiotic preparations. Italian Journal of Animal Science  volume 10:e31.
[28]  Nickolova M, Penkov D., 2004- Experimental influence of Laktina probiotic on egg laying characteristics, fertility and viability in muscovy duck (Cairina Moshcata). J Cent Eur Agric, 5, 353–358.