Isolation and diagnosis of Bacillus sp from the rhizosphere of halophytic wild plants and evaluation of the efficiency of isolates in dissolving Tri-Calcium Phosphate
Al-Qadisiyah Journal For Agriculture Sciences,
2021, Volume 11, Issue 2, Pages 37-43
AbstractBacillus sp was isolated and diagnosed from the rhizosphere of halophytic wild plants (Schanginia aeguptiace plant, Atriplex halimus plant, and the comparison soil far from the effect of the roots) and during three stages of plants growth (germination stage, vegetative growth stage, stage post flowering), and for three locations that included (Diyala University, Muqdadiya , Baladrooz) during the season of 2020. The diagnosis included studying the phenotypic, microscopic and biochemical features of these isolates and testing their efficiency in dissolving Tri-calcium phosphate Ca3(po4)2 as a source of phosphate after growing them in liquid and solid Pikovskayas media and estimating its solubility factor, and its efficiency in changing the degree of the medium reaction. The results of isolation and diagnosis showed the spread of Bacillus sp in saline environments, as 7 isolates out of 27 isolates that isolated during the growth stages of different halophytic wild plants were belonged to Bacillus subtilis, viz (B1, B5, B6, B7, B11, B15, B18, B19, B22, B23, B26), while the rest of the isolates belonged to Bacillus megaterium, whereas the isolates B1 and B2 were recorded the highest solubility factor reached (1.40 and 1.50) respectively, the isolate B26 was able to reduce the pH value of the inoculated medium to 4.81.
 M. Rashid, Khalil, S., Ayub, N., Alam, S., and Latif, A. (2004). Organic acids production and phosphate Solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions. Pak. J, Biol. Sci, 7: 187-197.
 S. A. A. Attia, Kazem L. A., Maid S. K., and Moaibed H. W . (2019). Study of some optimal conditions for the biological activity of Bacillus subtilis in degrading cellulose. Iraqi Journal of Science and Technology 10(2):1-7.
 E. Firew, Delelegn W., and Muleta, D.(2016). Phosphate Solubilization Potential of Rhizosphere fungi Isolated from Plants in jimma zon, southwest Ethiopia. Hindawi Publishing corporation International journal of Microbiology. Volume 2016, Article ID 5472601, 11.
 Abdul Ridha and Jabbar, A. K . (2015). Isolation and identification of Bacillus subtilis and testing its efficiency in dissolving solid phosphate in liquid cultures. Al-Muthanna Journal of Agricultural Sciences. 3(1): 1-7.
 Sundara, Rao W V B, Sinha M K.(1963). Phosphate dissolving microorganisms in the soil and rhizosphere. Indian j. Agric. Sci,33:272-288.
 N. A. Logan, and De Vos, P. (2009). Genus Bacillus in: Bergeys manual of systematic bacteriology, second Edition, vol. 3, springer Dordrecht Heidelberg London New York. pp: 121-128.
 S. D. J. Al-Sahlani, (2015). Isolation and identification of Bacillus cereus and its use in the production of polyhydroxybutyrate. PhD thesis, College of Agriculture and Food Sciences, University of Basra.
 M. A. Abdel Qader, and Sharif, A. Y . (2011). Isolation and identification of Bacillus species from locally prepared juices in the city of Mosul. Journal of Education and Science. 24(4): 28-36.
 Y. Chiu, Rekha, Wei, L., Aruna, A. (2006). Encapsulation of plant growth promoting bacteria in Alginate beads enriched with Humic acid. Wileyinter sci., 76-83.
 S. Donca, , Nimit, S. M.., Octavita E. A. (2005). Microbiological study of Bacillus megaterium strain with soil phosphors solubilzation potential. Paint Biol. Volumes, 49-50, p. 31-39.
 E. Koneman, Allen S., Janda W., and Winn W. (1997). "Color Atlas and Textbook of Diagnostic Microbiology". 5th ed., Lippincott-Raven Publishers, Philadelphia, USA.
 C.A. Black , (1965). Methods of soil analysis .part(1).physical and mineralogical soil properties An .Soc. Agrononmy. Inc. puplisher Madison, Wisconsin, USA.
 P. R. Murray, Baron, E. J., Jorgensen, J. H., Landry, M. L. and Pfaller M. A. (2007). Manual of Clinical Microbiology. 9th (ed). Washington, DC. American Cociety for Microbiology, USA.
 A. E. Brown, and Smith, H. R. (2017). Benson's Microbiological Applications, Laboratory Manual in General Microbiology 14th (ed). Mc Graw-Hill Higher Education. New York. 438pp.
 W. F. Harrigan, and McCance, M. E. (1976). Laboratory Methodsin Food and dairy Microbiology. Academic Press, London
 R. M. Smibert, and Krieg, N.R.(1981).General characterization .In: Sneath P.H. MURRAY,R.G.E., and costilow, R. N., Nester, E.W., Wood, W. A., Krieg, Philips, G, P.(eds). Manual of Methodes for general bacteriology: 409-443.
 E. J. Baron, and Finegold, S. M. (1990). Diagnostic microbiology. 8th. (ed). The C. V. Mosby company.
 K. A. Hassan, (2012). Isolation of phosphate-dissolving bacteria from soils and identification of organic acids produced from them. Iraqi Journal of Agricultural Sciences. 43(6): 72-77.
 M. Edi-premono, , Moawad, M. A., Vleck, P. L. G. (1996). Effect of phosphate Solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indonesian Journal of crop Science. 11, 13-23.
 A. T. Hikmat, (2019). Effect of some physical and chemical factors on the growth of Bacillus subtilis and the production of Xylanase enzyme. Journal of Education and Science. 28(1): 64-78.
 G. D. Saha, Chosh, P. A.,Isha, M.,and Saha, A.(2012). Isolation and of characteristincs tow new Bacillus subtilis strains from the rhizosphere of eggplant as potential biocontrol agents. J of plant pathology.94,1:109-118.
 H. A. S. Radi, , Abdul-Rida, H. A., and Hadwan., H. A. (2018). Isolation and identification of Bacillus megaterium from the rhizosphere of different crops, testing its efficiency in dissolving phosphate in liquid and solid culture media, and testing its growth with Bacillus subtilis and Bacillus mucilaginous bacteria as a fertilizer combination. Iraqi Journal of Soil Science. 18(1):214-223.
 Y. Andriani, , Rochimia. E, Ranayuning R. and Rtuee S,. (2017). Characterization of Bacillus megaterium and Bacillus mycoides Bacteria as probiotic Bacteria in fich and shrimp feed. In 2nd international conference on sustainabie Agriclture and food. Pag 127-135. Dol 10. 18502b/kls. V zi 6. 1029.
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