Preparation of Organic Selenocystine Using Locally Isolated Yeast
Al-Qadisiyah Journal For Agriculture Sciences,
2018, Volume 8, Issue 1, Pages 23-28
AbstractSelenium is a toxic inorganic form at very low concentration in life, while the organic-selenium compounds are appreciable interest and various of them have essential roles in nutritional science and cell biochemistry. Selenium-enriched yeast (Se-yeast) is a public form of selenium used to additional dietary intake of this essential trace mineral. In this study, an organic selenocystine by using locally isolated bakery yeast (Saccharomyces cerevisiae)is prepared. A novel locally prepared date extract media enriched by 0.2% potassium phosphate (KH2PO4), 0.6% ammonium sulfate is adopted as alternative culture media. Selenium salt is used in different concentrations (30, 60, 120 and 240 μg/mL), which are added to the yeast culture media. While the best concentration of selenium added is 30μg/mL, it achieves optimal conditions for the growth of red color yeast identical to the standard. The organic selenocystine is analyzed by High Performance Liquid Chromatography (HPLC) and Atomic Absorption Spectrophotometer (AAS) as compared with standard product obtained from Sigma. Results confirmed the formation of similar selenocystine products. .
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