Print ISSN: 2077-5822

Online ISSN: 2618-1479

Keywords : Asparaginase

Effect of Agricultural Exploitation on the Activity of Alkaline Phosphatase and Its Kinetic Properties in Some Soils

Luma Salih Jabbar Al-Taweel; Gaith Abedulkadhim Alwan Al- Jubouri

Al-Qadisiyah Journal For Agriculture Sciences, 2019, Volume 9, Issue 1, Pages 72-87
DOI: 10.33794/qjas.2019.162668

In order to study the role of agricultural exploitation in the activity of Alkaline phosphatase in the province of Diwaniyah, six sites that differ in some of their chemical, physical and biological properties were selected (Diwaniyah, Sunniya, Shamiya, Daghara, Afak and Al Budair). Three types of soils were chosen in each location, namely, orchard soil, field soil and jungle soil. The kinetic parameters of the enzymes maximum velocity (Vmax) and Michaelis constant (Km) were estimated in all study soils using increasing concentrations of the controlled substance. The results present that the highest efficacy of this enzyme is in the orchard soil of all sites except the location of the center Diwaniyah and Shamia. The average efficacy values ranged between (208.11 - 234.95) μg P-Nitro phenol. g-1 soil. 1 hour-1. The highest value of maximum velocity (Vmax) recorded at the field soil of Al-Shamia site (108.57) μg P-Nitro phenol. g-1 soil. 1 hour-1. The lowest value recorded at the soil of a jungle field at the Sunniya location (49.62) μg P-nitro phenol. g-1 soil. 1 hour-1. While the orchard soil of Al-Budair had the highest value of the Michaelis constant (Km) of (85.90) mM, the orchard soil of the Sunniya location had the lowest value (24.34) mM. .

The Effect of Fertilizers in Amidohydrolases enzymes in sunflower (Helianthus annuus) Rhizoshper

Luma .S.J. Al-Taweel

Al-Qadisiyah Journal For Agriculture Sciences, 2016, Volume 6, Issue 2, Pages 165-173
DOI: 10.33794/qjas.2016.115307

To study the enzymes activity for (Amidase , Urease , L-Asparaginase , L-Glutaminase and L-Aspartase) in sunflower plant rhizosphere in two different textures and the effect of the fertilizers kinds (mineral fertilizer , organic fertilizer and biofertilizer) in enzymes activity , two sites were chosen , the first was clayey silt texture and the second was sandy loam texture were fertilized in one level for every kind of fertilizers . The mineral fertilizer (160N kg.h-1 were added in two times before the cultivated and the second before flowering and 80 kg. P2O5 kg.h-1 were added before cultivated and 80kg K2O kg h-1 were added before cultivated). Organic fertilizer (poultry manure) was added as one level 10 Ton.h-1 and the bio fertilizer (Mikro-kondi) were added spraying 5 letter.h-1 in two times when start of vegetation about (6-8) leaves and the second spray before flowering about 4 weeks after the first treatment .
The results were summarized as :
• Increasing of enzyme activity for all enzymes in rhizosphere than the bulk soil.
• The enzymes activity in clayey silt texture were increased than the enzymes activity in sandy loam texture in all fertilized treatments.
• The organic fertilizer has high values than the author fertilizer kinds in enzymes activities and it arranged as follow :
Organic fertilizer  biofertilizer  mineral fertilizer  control
The highest enzymes activities values were in Amidase and the lowest were in L-Asperginase and it arranged as follow :
Amidase  L-Aspartase  Urease  L-Glutaminase  L-Asparaginase