Print ISSN: 2077-5822

Online ISSN: 2618-1479

Keywords : Key Words

A Study of The Degradation of Atrazin and 2,4-D Herbicide by Mutagenic Bacterial Isolates

Kareem Ubed Hasan

Al-Qadisiyah Journal For Agriculture Sciences, 2012, Volume 2, Issue 2, Pages 69-78
DOI: 10.33794/qjas.2012.68078

Microbial isolates are isolated from 25 agricultural soil samples contaminated by
herbicide. Their ability of degradation atrazin and 2,4-D herbicides are
examined . Two isolates are chosen according to ability for degradation , then
isolated and identified by using numbers of culture media and biochemical tests
Results have indicated that the bacteria are Escherichia coli (B6) and
Pseudomonas fluorescens (B13) , which have been incubated with a number of
herbicides concentrations (5.0 -17.5) mg L-1 from atrazin and (5.0 -20.0) mg L-1
from 2,4-D.
Results have shown the degradation increases with increasing the concentrations
(65 ، 60)% for isolate B6 , B13 respectively with 12.5 mg L-1 of atrazin and (73،
61)% of 2,4-D with 15.0 mgL-1 of atrazin and (73،61) % of 2,4-D with 15.0
mgL-1 after five days incubation.Chemical mutagen (Nitrosoguanidine) 500 μg
mL-1 used to mutant bacteria cell with time (0.5 , 1.0 , 1.5 and 2.0) hr. one hour
is enough to do that , there fore rasio degradation increases (93 , 89) % Atrazin
of (B13C6 , B6C3) respectively in 12.5 mgL-1 and (97 , 93) % , 2,4-D in 15.0
mgL-1 . Achridin orange and sodium Dodecyl sulphate (SDS) are used to curined
bacteria plasmids by 2500 μg mL-1 from (SDS) and 200 μg mL-1 from (Achridin
orange). The results show (B13C6 , B6C3) became curing cells.

The Effect of Ethanolic Extract of Melilotus officinal Flowers on Bleeding Time in Male and Female Rabbits

Hassan Toama Mohamed; Ali Sami Amen

Al-Qadisiyah Journal For Agriculture Sciences, 2012, Volume 2, Issue 2, Pages 15-20
DOI: 10.33794/qjas.2012.68153

The present study aimes to examining the effect of the ethanolic extract of
Melilotus officinalis flowers on bleeding time ,WBC and RBC count .Total
number in males and females adult rabbit eighteen, (9 males and 9females ) are
randomly divided into three equal groups (3 males and 3females per group). The
first (control) is drenched normal saline while the second and third groups are
drenched the ethanolic extracted (20 and 50mg/ml/kg of B.W) for 24 hours.
The results reveal significant increase in bleeding time in third group followed
by the second group compared with the control while RBC and WBC count
record no significant differences .On the other hand the gender had no
significant effect on the studied parameters.

Studying The Effect of Planting Method , Kind of Fertilizer on The Yield of Marjoram ( Majorana hortensis Moench. ) and Thyme ( Thymus vulgaris L. ) Plants

Saad Ali Ihsan

Al-Qadisiyah Journal For Agriculture Sciences, 2012, Volume 2, Issue 2, Pages 43-61
DOI: 10.33794/qjas.2012.68148

This research is conducted in a private field at Abu – Gharak , Babylon Province
, during two seasons ( 2009 & 2010 ), to study the effect of two planting
methods and kind of fertilizer on the yield , essential oil quantity in Marjoram
and Thyme plants .
Two field experiments are conduct by using randomized complete block design
(RCBD) with three factors in are replicates , factor (A) represent planting
method , (B) Nitrogen fertilizer , and (C) Iron fertilizer . The experimental units
represented by (one plot & four furrows ) . The Volatile oil extracted from theleaves and the flowers tops by hydro distillation method using Clevenger
apparatus , the volatile oil samples from the two plants were analyzed by (GLC).
The results obtain indicated that , there are significant effect for the fertilization
with Nitrogen and Iron on the growth , volatile oil quantity and percentage of
some oil components of Marjoram plants during the two seasons . Also foliar
application with Nitrogen & Iron elements produced high quantities of dry
matter and volatile oil in Marjoram plants that grown in furrows during the two
seasons , in addition , that foliar application with nitrogen raised Sabenene &
Terpenene percentages in the Marjoram oil .
The results during (2009 ) show that foliar application of nitrogen on Thyme
plants grown in plots with soil application with Iron produced high quantity of
dry matter and high volatile oil content comparing with the control .
In (2010) the results indicate that foliar application with nitrogen and iron to
Thyme plants that planted on furrows significantly affected the dry matter . In
addition , that high quantity of volatile oil produced in thymus plants due to the
foliar application with nitrogen and iron , also this application raised the two
aromatic compounds ( Thymol & Carvacrol ) percentages in Thyme oil