Print ISSN: 2077-5822

Online ISSN: 2618-1479

Keywords : Amidase


Salinity and Compost Amendment Impact on Amidase Activity in Rhizoshere of Tomato and Bean plants.

Luma S.J.Al-Taweel; Radhi K.Al-Rashidi

Al-Qadisiyah Journal For Agriculture Sciences, 2017, Volume 7, Issue 2, Pages 1-13
DOI: 10.33794/qjas.2017.162612

The aim of this study was to disclosed the activity of extracellular enzyme (amidase) in vertical and horizontal developing directions of rhizosphere (0-5 and 5-10) cm . The plots experiment was performed in Agriculture college / Al-Qadissiyah University for the spring season 2013 ,the sandy loam soil was used and cultivate Tomato (Lycopersicon Esculentum mill) and Bean (Phaseolus Vulgaris L.) plants under salinity levels (without salinity , 6 , 8 and 10) dS.m-1 and compost levels (0,10 and 20)T.h-1 , and their interactions completely randomized design (CRD) was used with six replicates, and the means of treatments were compared by L.S.D test 5% level .
The results were summarized as :
1- The enzyme activity were increased with increasing of the adding compost level 20Ton.h-1 and its interactions with salinity levels .
2- The enzymes activity were varied in rhizosphere development directions and the plants were differed in the site of highest activity , the enzyme activity in horizontal rhizosphere development direction was higher than the vertical direction .
3- The enzyme activity in tomato rhizosphere was higher than bean rhizosphere in all rhizosphere vertical and horizontal directions .
4- The high inhibitor percent of enzyme activity was at the level of 10 dS.m-1 salinity and the lowest was at the 6 dS.m-1. The inhibition percent were increased in vertical direction of rhizosphere developing (5-10)cm in tomato plant. The adding compost levels were varied by increasing or decreasing of enzyme activity according to rhizosphere development and it sites . .

The Effect of Fertilizers in Amidohydrolases enzymes in sunflower (Helianthus annuus) Rhizoshper

Luma .S.J. Al-Taweel

Al-Qadisiyah Journal For Agriculture Sciences, 2016, Volume 6, Issue 2, Pages 165-173
DOI: 10.33794/qjas.2016.115307

To study the enzymes activity for (Amidase , Urease , L-Asparaginase , L-Glutaminase and L-Aspartase) in sunflower plant rhizosphere in two different textures and the effect of the fertilizers kinds (mineral fertilizer , organic fertilizer and biofertilizer) in enzymes activity , two sites were chosen , the first was clayey silt texture and the second was sandy loam texture were fertilized in one level for every kind of fertilizers . The mineral fertilizer (160N kg.h-1 were added in two times before the cultivated and the second before flowering and 80 kg. P2O5 kg.h-1 were added before cultivated and 80kg K2O kg h-1 were added before cultivated). Organic fertilizer (poultry manure) was added as one level 10 Ton.h-1 and the bio fertilizer (Mikro-kondi) were added spraying 5 letter.h-1 in two times when start of vegetation about (6-8) leaves and the second spray before flowering about 4 weeks after the first treatment .
The results were summarized as :
• Increasing of enzyme activity for all enzymes in rhizosphere than the bulk soil.
• The enzymes activity in clayey silt texture were increased than the enzymes activity in sandy loam texture in all fertilized treatments.
• The organic fertilizer has high values than the author fertilizer kinds in enzymes activities and it arranged as follow :
Organic fertilizer  biofertilizer  mineral fertilizer  control
The highest enzymes activities values were in Amidase and the lowest were in L-Asperginase and it arranged as follow :
Amidase  L-Aspartase  Urease  L-Glutaminase  L-Asparaginase

Effect of Salinity and Compost on Some Enzymes Activity in Rizhosphere of Bean and Tomato Plants

Luma S.J.Al-Taweel; Radi K.Al-Rashidi

Al-Qadisiyah Journal For Agriculture Sciences, 2016, Volume 6, Issue 1, Pages 37-57
DOI: 10.33794/qjas.2016.110161

The aim of this study is to discover the activity of extracellular enzymes (amidase , alkaline phosphatase and Arylsulphatase) in vertical and horizontal developing directions of rhizosphere (0-5 and 5-10) cm . The plot experiment is performed in college of Agriculture / Al-Qadissiya University for the spring season 2013 ,the loamy sand soil was used to cultivate Tomato (Lycopersicon Esculentum mill) and Bean (Phaseolus Vulgaris L.) plants under salinity levels (without salted the natural salinity) , 6 , 8 and 10 ds.m-1 and compost levels (0,10 ,20)T.h-1 , and their interactions randomized complete design (RCD) is used with three replicates used with L.S.D test 5% level .The laboratory experiment is performed to determine the all enzymes activity above for all treatments .

The results are summarized as :
1- Enzyme activity decreases with increasing the salinity levels for all enzymes used in this study and the salinity level 10 ds.m-1 is the lowest. The arrangement of activity values as
Alkaline Phosphatase  Amidase  Arylsulphatase
2- The enzyme activity increased with increasing the added compost level 20Ton.h-1 and its interactions with salinity levels , and it has positive role to reduce the negative effect of salinity and it increases the activity in all treatments as well as the dead plant treatments .
3- The enzymes activity varied in rhizosphere developing directions and the plants are different in the site of highest activity , the enzyme activity in horizontal rhizosphere developing direction is highest than the vertical direction .
4- The enzyme activity in Tomato rhizosphere is the higher than Bean rhizosphere in all enzymes without the alkaline phosphatase in all rhizosphere vertical and horizontal directions .

Calculate of Activation Energy and Temperature Coefficient of Amidase in Some Soils .

Radhi Kadhum; Luma Salih

Al-Qadisiyah Journal For Agriculture Sciences, 2012, Volume 2, Issue 2, Pages 62-68
DOI: 10.33794/qjas.2012.68157

Ten soils are chosen from different geographic sites from Iraq :Abu
Ghraib(Three soils) ,Al-Jadriya ,Dyala (Tow soils) , AL-Sulaimaniya,Al-Kut
,Al-Nasriya , Al-Dewniya they differ in their chemical and physical properties ,
to determine Kinetic values (activation energy and temperature coefficient ) of
amidase enzyme in soils . The ten soils are incubated at 10-60 C° of incubation
temperatures ,and amidase activity is determined at each degree to calculate
kinetic values temperature coefficient (Q10) and activation energy (Ea) in soils
.Results have shown that the increasing of temperature cause increasing
hydrolysis of formamide (Amidase activity ) ,and the results have shown Q10
values of soils are between 1.83-2.37 ,where as Ea values are between 59.17-
86.61 K Jol.mol-1 .